Composite
SV40-8-2

Part:BBa_K2446035

Designed by: Yijie Pan   Group: iGEM17_Fudan   (2017-10-21)


SV40_8_PIP promoter

This part (Sv40 8*PIP ) is a mammalian synthetic promoter (SynPro) based on Sv40 promoter (BBa_K2446052) and eight repeats of PIP binding sites . We inset the 8*PIP binding sites behind the Sv40 promoter. Sv40 promoter is a constitutive expression promoter from the simian vacuolating virus 40. In this way, we can use our mammalian synthetic transcription factors (SynTFs) which is a fusing protein PIP_DBD_(G4S) linker_NLS_KRAB. The PIP-DBD can bind to the 8*PIP binding sites specifically[1] and the KRAB domain can repress the expression of Sv40 8*PIP promoter [2]. The corresponding SynTF of Sv40-8_PIP is ZF_PIP_KRAB (BBa_K2446038).

Figure 1: The design of SynTF-SynPros.

Figure 1: The design of SynTF-SynPros.

The information of other SynTF-SynPros is showed in the table below.

SynTFs SynPros
Gal4-KRAB(TF-KRAB-1) BBa_K2446037 Sv40-UAS(Sv40-UAS) BBa_K2446036
ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035
ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 SV40_8_ZF_21-16 BBa_K2446030
ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 SV40_8_ZF_42-10 BBa_K2446025
ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028
ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 SV40_8_ZF_54-8 BBa_K2446029
ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 SV40_4_ZFHD1 BBa_K2446032


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] M. Fussenegger et al., Streptogramin-based gene regulation systems for mammalian cells. Nature biotechnology 18, 1203--1208 (2000).

[2] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).

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